Prevalence of Vancomycin and High Level Gentamycin Resistance in Enterococcus Isolates in a Tertiary Care Hospital

Enterococcus account for about 12 % of healthcare-associated infections (HAI) and ranks third most common multi-drug resistant pathogen causing HAI. Continuous monitoring of the antibiotics susceptibility pattern of Enterococcus species thus became inevitable for control of Hospital Acquired infection. Aim of the study was to determine the prevalence of high-level Gentamycin and Vancomycin resistance enterococci among the clinical isolates. All clinical samples (Urine, Blood, CSF and others) received in a microbiology lab for bacterial culture and sensitivity were screened for enterococcus, only Enterococcus isolates were selected for this study. Primary inoculation was done on Blood agar and Mac Conkey agar culture media and incubated for 18-24 hrs at 37°C aerobically. Bacterial growth on culture media was identified by colony morphology, gram staining and Vitek-2 automated bacterium identifying system. The prevalence of high-level gentamycin resistance in Enterococcus was 74.5 % and Vancomycin-resistant in Enterococcus was 21.56 %. The study concludes that increased prevalence of high-level gentamycin and vancomycin-resistant in enterococci observed that we need to focus on infection control and prevention majors