PENENTUAN PROFIL BIOAUTOGRAFI DAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN BIDARA (Ziziphus mauritiana Auct. non Lamk.) DENGAN METODE PENANGKAPAN RADIKAL DPPH

Bidara or known by the Latin Ziziphus mauritiana Lam. has been known to have several activities, including hepatoprotective activity, antidiabetes, immunomodulators, and antioxidants. In addition, there are studies that report that the ethanol extract of Z. mauritiana leaves has adaptogenic activity. A theory suggests that antioxidant activity contributes to the adaptogenic activity possessed by a plant. So in this research will be tested the antioxidant extract ethanol leaves Z. mauritiana and the determination of bioautography profile with TLC. The results showed that ethanol extract of Z. mauritiana leaf had antioxidant activity with IC50 of 59,52 ± 1,2 μg / mL. Compared with vitamin C which has an IC50 value of 2.73 ± 0.05 μg / mL, the ethanol extract of Z. mauritiana leaves has lower antioxidant activity. The bioautographic profile results show the 4th and 10th spots on the flavonoid test, the 12th and 15th spots on the saponin test and the 10th spot on the triterpenoid test alleged to have antioxidant activity.