Supplementation Mannan-Rich Fraction (MRF) and / or Combination with Probiotic-Enhanced Water Acidifier on Dietary Female Broiler at 28 days as Natural Growth Promoters (NGPs)

— The limitation of using antimicrobial began in 1997 when the European Union (EU) banned using avoparcin for growth promoters to the livestock. The chase continued, until the last phase when the EU-wide ban on AGPs in animal feed (poultry) took effect since 2006.The research purpose to carried out the possible effect of mannan-rich fraction (MRF) and/ or combination with probiotic enhanced water as natural growth promoters (NGPs) on performance, blood biochemistry. A total of 160 broilers (females) 1- d-old Arbor Acres broiler were divided among 4 dietary treatments with 4 replicate cages per treatments and 10 males broiler per cage assigning experimental units to treatments randomly. The chicken will sacrificed at (28days) (6 each chicken will sacrificed) with amount total 24 broiler sacrificed each periods. The first experiment method was used completely randomized design with 4 treatments and 4 replicates. The treatments used for research were dietary with T0 (control), T1 (basal feed + MRF (Actigen™) 800g/ 400g / 200 g), T2 (Drinking water + 2 ml / L Combination feed additive (Acid-Pak 4-way®)), T3 (basal feed + MRF (Actigen™) 800g/ 400g / 200 g+ Drinking water 2 ml / L Combination feed additive (Acid-Pak 4- way®)). The parameters observed were performance and blood biochemistry. The data would be statistically analyzed using the analyses of variance using one-way anova with Completely Randomized Design (CRD) in Minitab® 16th edition. The differences between the means of groups were separated by Duncan Multiple Range Test (P < 0.05). It concluded the addition of mannan-riched fraction and combination with probiotic enhanced liquid acidifier has not significantly different (P > 0.05) on growth performance, and blood biochemistry.