Supplementation Mannan-Rich Fraction (MRF) and / or Combination with Probiotic-Enhanced Water Acidifier on Dietary Male Broiler at 28 on Intestinal Micro Flora and Ratio of Lactobacillus / Coliform

According to newest regulation PERMENTAN/14/16/2017 Indonesia has banned the use of antibiotics on poultry. The using feed additive is one method to improve the quality of feed. The research purpose to carry out the possible effect of mannan-rich fraction (MRF) and/ or combination with probiotic enhanced water as natural growth promoters (NGPs) on intestinal micro flora and ratio of lactobacillus / coliform. A total of 160 broilers (males) 1-d-old Arbor Acres broiler were divided among 4 dietary treatments with 4 replicate cages per treatments and 10 males broiler per cage assigning experimental units to treatments randomly. The chicken will sacrificed at starter periods (28days) (6 each chicken will sacrificed) with amount total 24 broiler sacrificed each periods. The first experiment method was used completely randomized design with 4 treatments and 4 replicates. The treatments used for research were dietary with T0 (control), T1 (basal feed + MRF (Actigen™) 800g/ 400g / 200 g), T2 (Drinking water + 2 ml / L Combination feed additive (Acid-Pak 4-way®)), T3 (basal feed + MRF (Actigen™) 800g/ 400g / 200 g+ Drinking water 2 ml / L Combination feed additive (Acid-Pak 4-way®)). The parameters observed were intestinal micro flora and ratio of lactobacillus / coliform The data would be statistically analyzed using the analyses of variance using one-way anova with Completely Randomized Design (CRD) in Minitab® 16th edition. The differences between the means of groups were separated by Duncan Multiple Range Test (P < 0.05). To sum up the addition of mannan-riched fraction and combination with probiotic enhanced liquid acidifier has not significantly different (P > 0.05) intestinal micro flora and ratio of lactobacillus / coliform.